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2.3. Histological and Immunohistochemical Analysis

DC Daniela Cabibi
AG Antonino Giulio Giannone
AQ Alberto Quattrocchi
VC Vincenza Calvaruso
RP Rossana Porcasi
DG Domenico Di Grusa
AP Anna Maria Pavone
AC Albert Comelli
SP Salvatore Petta
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All sections from liver biopsies of the 47 patients were stained with Hematoxylin-Eosin, PAS-D, Sirius Red, and Shikata’s Orcein staining. As a histological selection criterion, only liver biopsies with a portal tract length between 1.5 and 10 cm were included in the study.

Immunohistochemical (IHC) staining was performed with the automated Ventana BenchMark Ultra staining system (Ventana/Roche Tissue Diagnostics, Tucson, AZ, USA) according to the instructions of the manufacturers, using the following pre-diluted primary antibodies: anti-cytokeratin 7 (CK7, clone SP52; rabbit monoclonal; Ventana/Roche Tissue Diagnostics, Tucson, AZ, USA), anti-cytokeratin 19 (CK19, clone A53-B/A2.26; murine monoclonal; Cell Marque, Rocklin, CA, USA), and anti-EpCAM (Ber-EP4 clone; murine monoclonal; Cell Marque, Rocklin, CA, USA).

Copyright and License information:  ©2024 by the authors.
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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