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Enzyme-linked ImmunoSorbent Assay (ELISA) for PfHRP2

HN Hamtandi Magloire Natama
DO Delwendé Florence Ouedraogo
HS Hermann Sorgho
ER Eduard Rovira-Vallbona
ES Elisa Serra-Casas
MS M. Athanase Somé
MC Maminata Coulibaly-Traoré
PM Petra F. Mens
LK Luc Kestens
HT Halidou Tinto
AR Anna Rosanas-Urgell
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Detection of PfHRP2 antigen was performed with Malaria Ag CELISATM kit (Cellabs, Brookvale, New South Wales, Australia) following manufacturer’s recommendations. Briefly, 100 µL of umbilical cord plasma were tested and optical density (OD) read at 450 nm with MultiskanTM FC Microplate Photometer (Thermo Scientific). All samples and controls were tested in duplicate. Positive and negative controls were provided by the manufacturer and OD values were normalized using a reference positive control in all plates. Samples with a mean OD value above the cut-off level (OD of negative control +0.2 unit) were considered positive for PfHRP2 antigen as recommended by the manufacturer. The antigen index values were determined by dividing the mean OD value of each sample by the cut-off value as previously described55.

Copyright and License information: The Author(s) ©2017
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