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Genotyping

RJ Rong Jiang
YX Yuan Xu
PW Pan Wang
XC Xi Cheng
TS Tingyan Shi
RZ Rongyu Zang
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An additional 10 ml of venous blood sample (collected by the Tissue Bank of FUSCC after the diagnosis and before the initiation of treatment) were kept frozen till DNA extraction for genotyping. According to the standard protocol, genomic DNA was extracted from peripheral blood samples. Genotyping was performed using iPLEX MassARRAY platform (Sequenom, Inc., San Diego, CA, USA) as reported previously (Chen et al., 2014). Five percent of the samples were randomly selected for repeated genotyping, and the results were 100% concordant.

Copyright and License information:  ©2017 Jiang et al.
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.

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