2.9 Statistical analyses

For supernatant experiments, all conditions (5 MDM donors, 3 iMg lines) were replicated across three wells per experiment and repeated across 2–3 neuronal differentiations. All NMDA and ART treatments were replicated across three wells per experiment and at least 3 neuronal differentiations. Cell counts and area were assessed using unpaired t-tests (2 groups) or one-way ANOVA (3 or more groups). For longitudinal MEA experiments, one-way ANOVA was used to capture changes in neural activity over time, as opposed to assessing individual time points. For ART MEA experiments, all doses were individually compared to Veh using one-way ANOVA with Dunnett’s correction for multiple comparisons.