Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Investigation of the toxic potential of EB211 and EB279 in healthy mice

EB Eilnaz Basardeh
SP Somayeh Piri-Gavgani
HM Hamid Reza Moradi
MA Masoumeh Azizi
PM Parastoo Mirzabeigi
FN Farzaneh Nazari
MG Mostafa Ghanei
FM Fereidoun Mahboudi
FR Fatemeh Rahimi-Jamnani
request Request a Protocol
ask Ask a question
Favorite

To investigate the possible cytotoxic effects of EB211 and EB279, female C57BL/6 mice (groups of six, seven to eight-week-old), obtained from the Animal Laboratory of the Pasteur Institute of Iran, were injected intraperitoneally twice daily with EB211 (12 mg/kg), EB279 (12 mg/kg), or a cocktail of the two scFvs (6 mg/kg of each) for 72 h. Mice injected intraperitoneally twice daily with 30 mg/kg of CMS (Colistin is administered as the prodrug colistimethate sodium [CMS] [55–58]; Sigma-Aldrich, Saint Louis, USA) or NS for 72 h were used as the controls. All mice were sacrificed by intraperitoneal injection of an overdose (5 times the anesthetic dose; [59]) of ketamine and xylazine (Alfasan, Woerden, The Netherlands) at 72 h of infection. The kidneys and liver were aseptically removed and then fixed in 10% formalin for 24 h, followed by embedding in paraffin. Then, thin sections of tissues stained with hematoxylin-eosin were microscopically analyzed for histopathological changes.

Copyright and License information: The Author(s) ©2024
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

0/150

tip Tips for asking effective questions

+ Description

Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.

post Post a Question
0 Q&A