Figure 2e top: 5XUAS-CD4::tdGFP, nSybE-nlgSNTG4/nSybE-nlgSNTG4; orco-LexA::VP16/LexAop-nSyb::CD19
Figure 2e bottom: 5XUAS-CD4::tdGFP/CyO; orco-LexA::VP16/LexAop-nSyb::CD19, GH146-nlgSNTG4#4
Figure 3a top: 5XUAS-CD4::tdGFP/GMR17H02-LexA; LexAop-nSyb::CD19/GH146-nlgSNTG4#4. We noticed that the male R17H02 has lower LexA activity in DA1 than the female. Therefore, only the female flies were analyzed.
Figure 3a bottom: 5XUAS-CD4::tdGFP/GMR28H10-LexA; LexAop-nSyb::CD19/GH146-nlgSNTG4#4
Figure 3b top: 5XUAS-CD4::tdGFP/GMR17H02-LexA; LexAop-CD19::Sdc/GH146-nlgSNTG4#4. Only the female flies were analyzed.
Figure 3b bottom: 5XUAS-CD4::tdGFP/GMR28H10-LexA; LexAop-CD19::Sdc/GH146-nlgSNTG4#4
Figure 4a 5XUAS-CD4::tdGFP/GMR28H10-LexA; LexAop-nSyb::CD19/GH146-nlgSNTG4#4, LexAop-nSyb::CD19
Figure 4b and d 5XUAS-CD4::tdGFP/GMR28H10-LexA; LexAop-CD19::Sdc/GH146-nlgSNTG4#4
Figure 5b top: nSyb-nlgSNTG4, 5XUAS-CD4::tdGFP/nSyb-nlgSNTG4; LexAop-nSyb::CD19/LexAop-nSyb::CD19
Figure 5b bottom and 8: nSyb-nlgSNTG4, 5XUAS-CD4::tdGFP/nSyb-nlgSNTG4, pdf-LexA; LexAop-nSyb::CD19/LexAop-nSyb::CD19.
All crosses were maintained in a 25C incubator with 12 hr-12hr dark-light cycles, and were repeated at least three times.
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