2.9. Statistics

Data were analyzed in a completely randomized block design using SAS (SAS Inst. Inc., Cary, NC, USA). Steer was the experimental unit. Data were analyzed using the mixed procedure with block and treatment (PW0, PW40, PW80, and PW100) in the model. The percentage data for Choice carcasses in each treatment were analyzed via Chi-squared analyses. For ultrasound measures collected over time, the GLIMMIX procedure was used, and the model included block, treatment, time of measurement, and two-way interaction between time and treatment. Least square means were generated and separated using a protected least significant difference test. Significance was determined at p < 0.05.