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Hela, Siha, Hacat, and 293FT cells were cultured in 6-well plates overnight at a density of 2 × 105 per well, then HPV16 PsVs was added and cultured at 37 °C for 72 h, so that plasmids p16L1L2 and pCLucf can enter into cells and express HPV 16 L1 protein and EGFP.

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