Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Concise Method
2.5.1 Selection of the optimum donor beads concentration
Request a Protocol
Ask a
question
Favorite
The standard dilution solution was utilized to dilute the donor microspheres conjugated to capture antibodies to various concentrations (5, 10, 25, 50, 75, and 100 ng/mL), whereas the concentration of the acceptor microspheres conjugated to detecting antibodies was temporarily set at 75 ng/mL. The signal value of the high-concentration standard was detected.
Copyright and License information: ©2024 Fu, Sun, Qin, Zheng, Zhou, Zhou, Zhao, Xu and Huang.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
