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On the eighth day of the experiment, 10 mL of the culture was centrifuged at 10,000× g rpm for 5 min at 4 °C. The supernatant was then extracted, and the COD, NH3–N, TN and TP were measured using a Hach water quality analyzer [DR6000, Hach Water Quality Analysis Instrument (Shanghai) Co., Ltd., Shanghai, China].
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