Cell transfection and lentivirus infection

Briefly, miR-377-3p mimic, miR-377-3p inhibitor, and respective negative control (NC mimic, NC inhibitor) were purchased from Shanghai GenePharma Company (Shanghai, China). According to the manufacturer’s protocol, transfected them to cells with Lipofectamine 3000 (Invitrogen, Carlsbad, CA, USA). Lentiviruses loaded with shRNA were obtained from Shanghai GeneChem Company (Shanghai, China), including overexpressed METTL3 (Lenti-METTL3), sh-METTL3, overexpressed NEAT1 (Lenti-NEAT1), sh-NEAT1 and their corresponding lentiviral scramble control shRNA or their corresponding overexpression negative control vector (Lenti-NC). Lentivirus infection was performed according to the manufacturer’s instructions. The sequences above are shown in Supplementary Table 1. After transfection or lentiviral infection, cells were treated with or without 10 μM of BBR and then subjected to subsequent OGD/R treatment to simulate the brain ischemia-reperfusion environment in vitro.