Sedentary time and physical activity levels

Sedentary time and physical activity levels were objectively measured with a wrist-worn accelerometer (ActiGraph GT3X+, Pensacola, FL) for 7 consecutive days. The accelerometers were initialized to store raw accelerations at a sampling frequency of 100 Hz.³⁰ The raw accelerations were exported and converted to the “.csv” format using ActiLife, version 6.13.3. software (ActiGraph). The raw “.csv” files were then processed using the GGIR package (version 1.6–0; https://cran.r-project.org/web/packages/GGIR/) in R (version 3.1.2, https://www.cran.r-project.org/). Sedentary time and physical activity (light, moderate, and vigorous) levels were estimated as reported elsewhere.³¹