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To compare the relative efficacy of DMSO with its metabolites, DMS and DMSO2, on modulating inflammatory responses, the 3 compounds were analyzed in the whole blood assay as described above. The three compounds were dissolved in autologous plasma instead of PBS to increase the solubility of DMS in the whole blood assay. Also, to prevent the volatile DMS from affecting the evaluation of other treatments (DMSO and DMSO2), the 96 well plate was sealed with a plate sealer during the 7 h incubation period.
Copyright and License information: ©2016 Elisia et al
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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