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2.14. Gaussia luciferase assay
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To monitor tumor burden in vivo, blood samples were obtained weekly from the saphenous vein starting 1 week after ID8-p53−/−GLuc injection into C57BL/6 mice and until 5 weeks after tumor cell injection. Blood was centrifuged at 2,000 rpm for 15–20 min at room temperature, and plasma was recovered and stored at −80°C. Once thawed, 3 μl of every sample was diluted in PBS containing 0.1% BSA. Gaussia luciferase level in each sample was quantified by exposure to Coelenterazine-SOL (Nanolight) using a BioTek Synergy Mx plate reader and Gen5 2.07 Software.
Copyright and License information: ©2024 Rodriguez, Yakubovich, Murshed, Maranda, Galpin, Cudmore, Hanna, Macdonald, Ramesh, Garson and Vanderhyden
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