Right caudae epididymides were excised, rinsed with Dulbecco's Modified Eagle Medium (DMEM) medium (Gibco Rodano (MI), Italy), transferred on a Petri dish containing 1 mL DMEM medium and minced with scissors. Epididymal pieces were fluxed through a Pasteur pipette to facilitate sperm extrusion. Sperm suspension was filtered through a 200-mesh nylon to remove tissue fragments and diluted up to 10 mL. Sperm were counted by a Neubauer chamber under a light microscopy (Nikon Eclipse Ts2) [10].
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