MapInspect software (https://mapinspect.software.informer.com) was applied to map the chromosomal positions of the ADH genes in tobacco. Based on the annotation information and the full genome protein sequences of tobacco [30], the MCScanX [37] software was used to analyze the possible segmental duplication and tandem duplication events with default parameters, and the TBtools software [38] was used for visualization. The tobacco K326 genome database released by Edwards (2017, Nitab-v4.5) was used as reference genome (https://solgenomics.net/ftp/genomes/Nicotiana_tabacum/edwards_et_al_2017/).
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