The toxicity of cyhalothrin on female H. rufipes was determined by the impregnation method, and three sublethal concentrations (LC05, LC10 and LC50) of cyhalothrin were selected. The salivary glands, ovaries, midgut and Malpighian tubules of females that were treated with different sublethal concentrations were dissected as described above. Then the relative expression of HrGSTm1 in different tissues was determined using qPCR. Ticks without cyhalothrin treatment were used as the negative control.
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