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After 6 weeks of the Time-Restricted Exercise schedule, mice were allowed a single day of 24-h ad libitum access to an unlocked running disc (n = 9–10 mice/group). Wheel activity was recorded in 10-min bins throughout the 24-h period in 12:12 LD conditions. Wheel activity was presented in 1-h bins (Fig. 3A). Cosinor analysis was performed on data recorded every 10 min to determine activity acrophase for each mouse using Cosinor Online48, and compared between EAP and LAP mice to evaluate shifts in activity rhythms (Fig. 3B).
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