Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Request a Protocol
Ask a
question
Favorite
Cells were cultivated for 24 h. The harvested cells were washed with PBS and fixed with ice-cold 70% ethanol overnight. The fixed cells were resuspended in PBS (containing 0.2 mg/mL RNAse and 10 mM PI) and incubated in the dark for 30 min at 37 degrees. A FACSCalibur flow cytometer (Becton Dickinson, San Jose, CA) was used for flow cytometric analysis, and the results were analyzed using a correlation test.
Copyright and License information: The Author(s) ©2024
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
