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2.4. MALDI-MSI analysis
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Before MALDI-MSI, the instrument was calibrated by spotting the pep II calibration standard onto the matrix-coated ITO glass slide. All the mass spectra were automatically acquired over a mass range of m/z 1000 to 4000 in the positive reflection mode. Laser shots of 400 times were accumulated at one raster position and a raster step size of 100 µm.
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