Quantification of western blots

Statistical analyses were done with Prism6/7 software (GraphPad). Western blot protein bands were quantified using ImageJ/FIJI 2.0 using the Gels tool. A histogram was generated for each band where the peaks were proportional to the intensity of the band. The area under the curve was used as the quantitative value. Where necessary these bands were normalised to control bands. At least three biological repeats were done and averaged. For the comparison of two groups, a two-tailed t-test was used.