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Acridine orange/ethidium bromide (AO/EB) apoptosis assay
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The technique of AO/EB staining was used to identify apoptotic cells. In a 24-well plate (4 × 105 cells/well), ovine fetal BM-MSCs were cultivated and incubated for 72 h under various conditions. The cells were washed with DPBS, stained with 5 µL of the AO-EB working solution (100 µg/mL AO and 100 µg/mL EB in distilled water), and examined under a fluorescent microscope immediately53.
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