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Scanning transmission electron microscopy (STEM)

PP Pallabi Sil Paul
TP Tark Patel
JC Jae-Young Cho
AY Allan Yarahmady
AK Aria Khalili
VS Valentyna Semenchenko
HW Holger Wille
MK Marianna Kulka
SM Sue-Ann Mok
SK Satyabrata Kar
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The morphological changes of 0N4R and 1N3R tau samples obtained from various experimental paradigms were evaluated using an ultra-high resolution Hitachi S-5500 cold field emission STEM. Initially 5 µL tau samples with or without Aβ seeds and PLGA were deposited onto plasma cleaned, carbon coated copper grids for 30 s, blotted to remove excess liquid, air-dried and then softly washed with 10 µL of Milli-Q water to remove the salt. The grids were subsequently stained with 2% aqueous uranyl acetate for 30 s, blotted to take off excess liquid, dried and then imaged at 30 kV accelerating voltage and 30 µA emission current as described previously29.

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