DNA was isolated from mouse skin using a Wizard genomic DNA purification kit (Promega, Madison, WI, USA) and quantified using a spectrophotometer. The levels of 8-OHdG present in DNA isolated from mouse skin tissues were assayed using a Bioxytech 8-OHdG-ELISA kit (OXIS Health Products, Portland, OR, USA), according to the manufacturer’s instructions.
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