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Group-housed silent males exposed to hums
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A second set of controls investigated the degree to which hearing a hum might contribute to activation of PAG neurons (n = 3). Two males were individually housed with an artificial nest identical to other experimental conditions in larger tanks split with a mesh divider (see Fig. 3A in 38). We remotely monitored each male with separate hydrophones placed near their respective nests, allowing us to ensure that the male hearing hums did not produce any vocalisations.
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