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Tissue microarray construction

XZ Xiaomei Zhang
LL Lin Li
FG Fuping Gao
BL Binbin Liu
JL Jing Li
SR Shuang Ren
SP Shuangshuang Peng
WQ Wei Qiu
XP Xiaohong Pu
QY Qing Ye
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Each tissue sample underwent immediate fixation in 10% neutral buffered formalin for a duration of 12–48 h, followed by paraffin embedding. The processed samples were subjected to routine deparaffinization and rehydration procedures. A tissue microarray (TMA) was created using the Grand Master automated arrayer (3DHISTECH Ltd., Budapest, Hungary), with 2 mm punch size obtained from representative tumor blocks of each case. The tumor core was extracted from the invasive front of the deepest tumor invasion portion, with avoidance of necrotic areas. Dual representations of TMAs were constructed and then sectioned into 4-µm-thick sections for histological, immunohistochemical, and FISH detection procedures. IHC and FISH were performed in both duplicates of each case, and in case of disagreement between the duplicates in FISH or IHC results, the entire slide was used for a final decision.

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