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Cell viability assay (MTT assay)

FM Fatemeh Mohajerani
ZT Zahra Moazezi Tehrankhah
SR Saeid Rahmani
NA Nastaran Afsordeh
SS Sajad Shafiee
MP Mohammad Hossein Pourgholami
BS Bahram M. Soltani
MS Majid Sadeghizadeh
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U87 and C6 cell lines (2000 cells/well) were seeded in 96-well plates in triplicate. After 24 h, the cells were transfected with pCL19A or pCDNA3.1( +) mock vectors (0.2 μg/well) using Lipofectamine3000 reagent (0.2 μL/well) (Thermo Fisher Scientific, USA). At 24 h, 48 h, 72 h, and 96 h post-transfection, 10 μL of 5 mg/mL Tetrazolium salt (Sigma, USA) were added to each well, followed by an additional incubation at 37 °C for 4 h. The cell supernatants were aspirated and discarded once the purple precipitate appeared in the wells. Next, 100 μL DMSO (Merk, USA) was added to each well to dissolve the formazan crystals followed by a measurement of optical density (OD) at 570 nm with an ELISA Microplate Reader (BioTek, USA).

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