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Bacterial strains and plasmids are listed in Table 2. E. coli MG1655 wild type (WT) strain was used as the parental strain for isogenic gene deletion and chromosomal modification. E. coli strain BL21 (DE3) was used for recombinant protein expression. Standard Luria–Bertani (LB) broth with appropriate antibiotics (100 mg/mL ampicillin, 50 mg/mL kanamycin, or 25 mg/mL chloramphenicol) was used for E. coli culture.
Bacterial strains and plasmids used in this study
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