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Cells were fixed with 100% methanol and permeabilized with 0.5% Triton X-100 in PBS. After blocking with 10% sheep serum in PBS for 30 min, the cells were incubated overnight at 4 °C with LC3A/B antibody (1:100). Nuclei were stained with DAPI for 5 min in the dark, and images were acquired under a fluorescence microscope.
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