Endothelial cell proliferation and migration assay

HL Hongwei Lv
QZ Qianni Zong
CC Cian Chen
GL Guishuai Lv
WX Wei Xiang
FX Fuxue Xing
GJ Guoqing Jiang
BY Bing Yan
XS Xiaoyan Sun
YM Yue Ma
LW Liang Wang
ZW Zixin Wu
XC Xiuliang Cui
HW Hongyang Wang
WY Wen Yang
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SVEC4-10 cells were seeded at a density of 3000–5000 cells per well in a 96-well plate. After indicated treatment for 24 h, cell viability was measured using Cell Counting Kit-8 (CCK-8) assay (Vazyme) following manufacturer’s instruction. For migration assay, 5 × 104 overnight serum-starved SVEC4-10 cells were seeded on the PET membrane (8 µm pore size) of an insert. The inserts were hanged on a 24-well support plate, which had a 70–80% confluent monolayer of cultured Hepa1-6-Ctrl or Hepa1-6-Cgas cells with complete growth media. After 24 h of incubation, the inserts were fixed with 4% paraformaldehyde and stained with crystal violet solution. Representative fields were photographed and the migrated cells were quantified by ImageJ software (NIH).

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