SVEC4-10 cells were seeded at a density of 3000–5000 cells per well in a 96-well plate. After indicated treatment for 24 h, cell viability was measured using Cell Counting Kit-8 (CCK-8) assay (Vazyme) following manufacturer’s instruction. For migration assay, 5 × 104 overnight serum-starved SVEC4-10 cells were seeded on the PET membrane (8 µm pore size) of an insert. The inserts were hanged on a 24-well support plate, which had a 70–80% confluent monolayer of cultured Hepa1-6-Ctrl or Hepa1-6-Cgas cells with complete growth media. After 24 h of incubation, the inserts were fixed with 4% paraformaldehyde and stained with crystal violet solution. Representative fields were photographed and the migrated cells were quantified by ImageJ software (NIH).
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