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MTS assay

CR Caterina De Rosa
VR Viviana De Rosa
CT Concetta Tuccillo
VT Virginia Tirino
LA Luisa Amato
FP Federica Papaccio
DC Davide Ciardiello
SN Stefania Napolitano
GM Giulia Martini
FC Fortunato Ciardiello
FM Floriana Morgillo
FI Francesca Iommelli
CC Carminia Maria Della Corte
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Drug-induced toxicity was assessed by using the MTS assay (Cell Counting Kit-8). Briefly, cells were seeded in 96-well flat-bottomed plates at a density of 1,000 cells/well and treated for 72 h with increasing concentrations (0.01–10 µM) of osimertinib, selumetinib, DNA-PK-I, AURK-A-I, PARP-I, ATM-I, berzosertib or ATR-I. The number of viable cells was determined spectrophotometrically and expressed as the percentage of viable cells, considering the untreated control cells as 100%. Concentration that inhibits 50% of cell growth (IC50) was determined by using AAT Bioquest online tools (https://www.aatbio.com/tools/ic50-calculator). At least three independent experiments were performed in triplicates and data were pooled.

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