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For the MTT assay, differentiated cells were incubated with 0.1–100 μM paclitaxel for 24 h in PC12 cells. A549 cells were incubated with 100 μM paclitaxel and either TJ24 or TJ68 for 24 h. After washing cells once with DMEM, they were incubated in 0.5 mg/mL MTT for 4 h, and then lysed in 500 μL DMSO. For each well, 200-μL supernatant was transferred to a 96-well plate, and absorbance at 570 nm measured using a microplate reader (TECAN InfiniteF50R) [8].
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Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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