For consistency, we used apamin purified from the honeybee Apis mellifera venom (product number A1289) and synthetic melittin (M4171) purchased from Sigma-Aldrich. TRAM-34 was synthesized as described (Wulff et al., 2000). Other low-molecular-weight compounds were purchased from Sigma-Aldrich: acetylcholine chloride (ACh; A6625), capsaicin (M2028), capsazepine (C191), glycine (G7126), kainic acid monohydrate (K0250), and N-methyl-D-aspartic acid (NMDA; M3262).
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Tips for asking effective questions
+ Description
Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.