BALF was performed to determine the total cell numbers using a hemocytometer. Smears of BALF cells were stained with Giemsa to determine the neutrophil counts. The concentrations of IL-17 (R&D Systems,USA), IL-22 (R&D Systems,USA),TNF-α (R&D Systems,USA), IL-6 (Neobioscience Technology Co., Ltd, China) and MPO activity (Colorful Gene, China)in the BALF were measured with enzyme immunoassays, according to the manufacturer’s protocols.
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