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Plant material and growth conditions

XZ Xiaohui Zhang
TL Tongjin Liu
XL Xixiang Li
MD Mengmeng Duan
JW Jinglei Wang
YQ Yang Qiu
HW Haiping Wang
JS Jiangping Song
DS Di Shen
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B. oleracea var. alboglabra 4E286 is a cytoplasmic male sterile line. The B. rapa L. var. purpurea HCT3 is an inbred line. The seeds of these plants and their progeny were surface-sterilized in 1% NaClO and germinated on glass Petri dishes at 25 °C. After germination, seedlings were transplanted into a mixture of peat soil (peat:moss:perlite:vermiculite soil = 3:2:1:1). The seedlings were transplanted to a greenhouse after 2 weeks and were watered and fertilized regularly.

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