Isoprenaline hydrochloride (Iso), sodium nitroprusside dehydrate (SNP) and ethyl 3-aminobenzoate methanesulfonate (MS-222, tricaine) were purchased from Sigma Aldrich. Iso is a β1/β2-adrenoreceptor agonist [15,25,26], whereas sodium nitroprusside (SNP) is a potent nitric oxide donor. Nitric oxide plays a major role in regulating the vascular diameter, endothelial cell migration and angiogenesis [27,28,29,30]. The paralytic agent μ-conotoxin GIIIB was obtained from Alomone Labs, Israel.
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