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RT-PCR Analysis and quantitative Real Time PCR

EC Elisa Cirelli
ED Emanuela De Domenico
FB Flavia Botti
RM Renato Massoud
RG Raffaele Geremia
PG Paola Grimaldi
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Total RNA was extracted from Sertoli cells using Trizol reagent (Invitrogen) according to the manufacturer’s instructions and 1 μg was used for retrotranscription (RT) using M-MLV reverse transcriptase (Invitrogen). cDNA produced by the RT reaction was used as template for semiquantitative PCR analysis (GoTaq, Promega) or quantitative Real Time PCR performed using SSOADV Universal SYBR Green (BioRad) in a PRISM 7300 Sequence Detection System (Applied Biosystems). All the primers used are listed in Table 1.

List of primers.

Copyright and License information: The Author(s) ©2017
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