Plating assays

C. albicans strains were grown overnight at 30°C in S-Raffinose (2% raffinose, 6.7% Yeast Nitrogen Base with ammonium sulfate and a full complement of amino acids). Strains were diluted ~100-1000 fold in the morning and grown for 4-6 hr. Strains were serially diluted 10-fold into S-Raffinose five times and ~5 µl of cells from each dilution were spotted onto SD (2% Dextrose, 6.7% Yeast Nitrogen Based with ammonium sulfate and amino acids), S-Galactose (2% Galactose, 6.7% Yeast Nitrogen Based with ammonium sulfate and amino acids) plates ± 3 µg/ml Antimycin A (Millipore Sigma, St. Louis, MO), or S-GlcNAc (2% Galactose, 6.7% Yeast Nitrogen Based with ammonium sulfate and amino acids) plates. Strain growth was monitored visually; strains that grew well exhibited growth across all dilutions. We note that Y. lipolytica is an obligate respirator; hence, Antimycin A was not included in any Y. lipolytica plating assays.