Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Ask a
question
Favorite
C4-2, CWR22Rv1(22Rv1), VCaP and the 293T cell lines were obtained from American Type Culture Collection. C4-2, 22Rv1 and VCaP cells were maintained in RPMI 1640 medium and 293T cells in DMEM media, all containing 10% fetal bovine serum (FBS), antibiotics (100 units/ml penicillin and 100 µg/ml streptomycin), and 2 mM glutamine (Invitrogen) in a humidified 5% CO2 environment at 37 °C. All cell lines were characterized and authenticated as bacteria and mycoplasma free following ATCC’s instructions.
Copyright and License information: The Author(s) ©2022
Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
