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To estimate the divergence time among these 11 species, all of the 4DTv sequences were extracted from the previous super sequences using Reseqtools (https://github.com/BGI-shenzhen/Reseqtools). MCMCtree program in the PAML package (v4.8) (Yang 2007) was used for divergence time calculation. The divergence times were analyzed by Markov chain Monte Carlo sampling with samples drawn every 2,000 steps and 100,000 samples, and the results were calibrated by the fossil records downloaded from the TIMEtree database (http://www.timetree.org).
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