Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Heparin column chromatography

EL Emmanuelle V. LeBlanc
CC Che C. Colpitts
request Request a Protocol
ask Ask a question
Favorite

VSV-SARS-CoV-2 (105 PFU) was loaded onto a 1-mL HiTrap heparin column (Cytiva 17040601) in 10 mM sodium phosphate (pH 7.4). The column was washed with 5 mL of the same buffer and eluted with heparin, EGCG, N-acetylneuraminic acid in the same buffer. Alternatively, pre-treated VSV-SARS-CoV-2 was loaded and washed with the same buffer. Still-bound virions were then eluted with 2 M NaCl in 10 mM sodium phosphate (pH 7.4). Fractions were concentrated using Amicon 100 K ultrafiltration tubes and analyzed for viral RNA.

Copyright and License information: The Author(s) ©2022
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

0/150

tip Tips for asking effective questions

+ Description

Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.

post Post a Question
0 Q&A