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2.3. Platelet granule secretion and calcium mobilisation measurements
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P‐selectin exposure in human and mouse platelets was measured by flow cytometry and calcium mobilisation in human platelets assayed as described previously using Fura‐2 AM (Holbrook et al., 2018). ATP secretion from dense granules was measured using lumi‐aggregometry (Holbrook et al., 2012).
Copyright and License information: ©2020 The Authors. British Journal of Pharmacology published by John Wiley & Sons Ltd on behalf of British Pharmacological Society
This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
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