2.3. Construction of Green Fluorescent Protein (GFP) Vector

In order to construct a FgFly1-GFP vector, the PCR product and the pYF11-GFP-GEN vector digested by XhoI were cotransferred into yeast XK1-25, and the yeast plasmid pYF11-FgFly1-GFP-GEN was obtained. The yeast plasmid pYF11-FgFly1-GFP-GEN was extracted and transformed into E. coli DH5α for large-scale amplification of the recombinant plasmid [39]. The FgFly1-GFP fusion vector was transformed to observe the localization of FgFly1. The transformed complementary strains were screened and inoculated on PDA medium with 100 mg/L G418 sulfate. The corresponding primers of the complementary strains were analyzed by PCR and Southern blot. Finally, the green fluorescent protein (GFP) of the obtained strain was observed with a confocal microscope.