ARPE-19 cells were obtained from the American Type Culture Collection (Manassas, VA, USA) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, low glucose formulation, 11330-033; Gibco, Invitrogen, CA, USA) supplemented with 10% (v/v) foetal bovine serum (FBS, 04-001-1ACS; BI, US origin), 100 U/ml penicillin and 100 μg/ml streptomycin (10378-016; Gibco).
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