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Apoptosis assays

JZ Jian Zhang
YL Yao Lu
YM Yangming Mao
YY Yue Yu
TW Tianyu Wu
WZ Wei Zhao
YZ Yeqian Zhu
PZ Pengcheng Zhao
FZ Fengxiang Zhang
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H9c2 cells were seeded in 1 × 105/6-well tissue culture plates overnight and treated with the different Exos or PBS before OGD. The cells were washed with PBS and stained with Annexin V fluorescein isothiocyanate and propidium iodide apoptosis kit (40302ES20, YEASEN, China). Flowjo Software version 10.0 (Tree Star, USA) was used to analyze apoptotic cells. TdT-mediated dUTP Gap End Labeling (TUNEL) Apoptosis Detection Kit (Roche, USA) was also used to detect cell or tissue apoptosis according to the manufacturer's instructions. The formula for calculating the percentage of apoptotic nuclei was the total number of nuclei divided by the total number of TUNEL-positive nuclei.

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