Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Single Locus Tracking

HK Hyeyeong Ku
GP Gunhee Park
JG Jiyoung Goo
JL Jeongmin Lee
TP Tae Lim Park
HS Hwanyong Shim
JK Jeong Hee Kim
WC Won-Ki Cho
CJ Cherlhyun Jeong
request Request a Protocol
ask Ask a question
Favorite

Image analysis was performed using ImageJ scripts and custom-built MATLAB codes for mean square displacement calculation. Single locus tracking was performed by an ImageJ plugin-in software, TrackMate. Each fluorescent locus per frame was identified by Laplacian Gaussian fitting with an LoG detector in the TrackMate. Background noise was minimized by the LoG detector which is based on applying an LoG filter on each image and detecting local maxima. 2D trajectories of X-Y coordinates over time for each particle were obtained.

Copyright and License information:  ©2022 Ku, Park, Goo, Lee, Park, Shim, Kim, Cho and Jeong.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A