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Caspase 3/7 Assay

SS Seda Seren
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JJ Jean-Patrick Joly
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PV Pierre Voisin
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VB Véronique Bouchaud
GA Gérard Audran
SM Sylvain R. A. Marque
PM Philippe Mellet
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The Caspase-Glo 3/7 Assay (Promega) was used according to the manufacturer’s instructions. The assay contains a luminogenic caspase 3/7 substrate in a reagent optimized for caspase and luciferase activity. Addition of the reagent on cell medium results in cell lysis followed by caspase cleavage of the substrate and then the action of luciferase to generate a luminescence signal which is proportional to the amount of caspase activity. The assay protocol was started by equilibrating of cell plate and caspase substrate at room temperature. Next the reagent was added in each well of plate, and the plate was incubated 15 min at room temperature. The luminescence was recorded using the Varioskan LUX multimode microplate reader (Thermo Scientific).

Copyright and License information:  ©2022 The Authors. Published by American Chemical Society
Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/).

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