2.10. Cell Growth and Cell Proliferation Analyses

Cell growth curves were assessed by using the Cell Counting Kit-8 (CCK-8) (Abmole, Houston, TX, USA). Cells were seeded at a density of 2000 cells per well in 96-well plates and incubated for 1, 2, and 3 days, respectively. Then, the cells were incubated with CCK-8 solution for 2 h. The optical density (OD) values were measured on the microplate reader (Infinite M200 Pro, Tecan Group, Männedorf, Switzerland) at a wavelength of 450 nm. Similarly, cell proliferation was quantified on day 2 using a 5-bromo-2-deoxyuridine (Brdu) Cell Proliferation Assay Kit (BioVision, Milpitas, CA, USA).