Infection of HEK 293T cells

Fifty thousand cells seeded in 48-well plates were incubated in the presence of rAAV2/1 at a multiplicity of infection of 10³ vg/cell in serum-free medium containing human adenovirus 5 (H5) reference material (ATCC VR-1516; titer 5.8 × 10¹¹ particle forming units (pfu)/ml) (LGC Standards, Teddington, Middlesex, UK) at the multiplicity of infection of 50 particle forming units (pfu)/cell. After 2 hours, the cells were rinsed with serum-supplemented medium and further cultured for 72 hours without passaging in culture medium containing or not Dox at the indicated concentration.