Taqman PCR-Array and Taqman qRT-PCR

Expression changes were analysed by quantitative Taqman-RT-PCR [16]. For analysis of expression changes caused by O. regalis extract in invading cells, HLaC78 spheroids were grown and transferred to laminin coated 96 well plates as described above. After 18 h invasion with or without plant extract, spheroids and invaded monolayer cells were harvested by trypsination and pooled. RNA was isolated (see above). To evaluate gene expression changes, Taqman array plate for cell adhesion molecules (Applied Biosystems, Darmstadt, Germany) were supplied with cDNA, reverse transcribed with the high capacity RNA to cDNA Master Mix (Applied Biosystems, Darmstadt, Germany) according to the manufacturers protocol. Taqman qRT-PCR was carried out according to the manufacturer’s instructions for MDR-1.